One of my favorite Humble Beginning moments appeared in the very first Spider-Man story from Amazing Fantasy #15 (1962). For the most part, the costume created by young Peter Parker looked exactly as it does today some 46 years later...but toward the end of the 11-page story, a peculiar new detail appears in this panel:
Those beady little eyes peeping through the mask's white "eye" shapes made their first and last appearance here in this panel...which I can back up with the same panel from the page's original art (which was recently donated to the Library of Congress):
Why the eyes? My guess is that artist Steve Ditko added them to amp up Peter Parker's shock at recognizing the burglar who killed his Uncle Ben. I have to admit, though...as jarring as the dot-eyes look, when subsequent reprints removed them, the scene lost much of the emotional impact those odd little dots imparted.